Bio-based communication networks could control cells in the body to
treat conditions

Date:
August 17, 2020
Source:
American Chemical Society
Summary:
Electronic devices and biological cells communicate through very
different mechanisms. Now, scientists report progress on tiny
communication networks that overcome this language barrier, allowing
electronics to eavesdrop on cells and alter their behavior -- and
vice versa. Potential applications include a capsule that could
be swallowed to track blood sugar and make insulin when needed.



FULL STORY ==========================================================================
Like electronic devices, biological cells send and receive messages,
but they communicate through very different mechanisms. Now, scientists
report progress on tiny communication networks that overcome this
language barrier, allowing electronics to eavesdrop on cells and alter
their behavior -- and vice versa.

These systems could enable applications including a wearable device that
could diagnose and treat a bacterial infection or a capsule that could
be swallowed to track blood sugar and make insulin when needed.


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The researchers will present their results today at the American Chemical Society (ACS) Fall 2020 Virtual Meeting & Expo.

"We want to expand electronic information processing to include biology,"
says principal investigator William E. Bentley, Ph.D. "Our goal is
to incorporate biological cells in the computational decision-making
process." The new technology Bentley's team developed relies on redox mediators, which move electrons around cells. These small molecules
carry out cellular activities by accepting or giving up electrons
through reduction or oxidation reactions. Because they can also exchange electrons with electrodes, thereby producing a current, redox mediators
can bridge the gap between hardware and living tissue. In ongoing work,
the team, which includes co-principal investigator Gregory F. Payne,
Ph.D., is developing interfaces to enable this information exchange,
opening the way for electronic control of cellular behavior, as well as cellular feedback that could operate electronics.

"In one project that we are reporting on at the meeting, we engineered
cells to receive electronically generated information and transmit it as molecular cues," says Eric VanArsdale, a graduate student in Bentley's
lab at the University of Maryland, who is presenting the latest results
at the meeting.

The cells were designed to detect and respond to hydrogen peroxide. When
placed near a charged electrode that generated this redox mediator, the
cells produced a corresponding amount of a quorum sensing molecule that bacteria use to signal to each other and modulate behavior by altering
gene expression.

In another recent project, the team engineered two types of cells to
receive molecular information from the pathogenic bacteria Pseudomonas aeruginosa and convert it into an electronic signal for diagnostic and
other applications. One group of cells produced the amino acid tyrosine,
and another group made tyrosinase, which converts tyrosine into a molecule called L-DOPA. The cells were engineered so this redox mediator would be produced only if the bacteria released both a quorum sensing molecule and
a toxin associated with a virulent stage of P. aeruginosa growth. The
size of the resulting current generated by L-DOPA indicated the amount
of bacteria and toxin present in a sample. If used in a blood test, the technique could reveal an infection and also gauge its severity. Because
this information would be in electronic form, it could be wirelessly transmitted to a doctor's office and a patient's cell phone to inform
them about the infection, Bentley says. "Ultimately, we could engineer
it so that a wearable device would be triggered to give the patient a therapeutic after an infection is detected." The researchers envision eventually integrating the communication networks into autonomous systems
in the body. For instance, a diabetes patient could swallow a capsule containing cells that monitor blood sugar. The device would store this
blood sugar data and periodically send it to a cell phone, which would interpret the data and send back an electronic signal directing other
cells in the capsule to make insulin as needed. As a step toward this
goal, VanArsdale developed a biological analog of computer memory that
uses the natural pigment melanin to store information and direct cellular signaling.

In other work, Bentley's team and collaborators including Reza Ghodssi,
Ph.D., recently designed a system to monitor conditions inside industrial bioreactors that hold thousands of gallons of cell culture for drug
production. Currently, manufacturers track oxygen levels, which are
vital to cells' productivity, with a single probe in the side of each
vessel. That probe can't confirm conditions are uniform everywhere
in the bioreactor, so the researchers developed "smart marbles"
that will circulate throughout the vessel measuring oxygen. The
marbles transmit data via Bluetooth to a cell phone that could adjust
operating conditions. In the future, these smart marbles could serve as
a communication interface to detect chemical signals within a bioreactor,
send that information to a computer, and then transmit electronic signals
to direct the behavior of engineered cells in the bioreactor. The team is working with instrument makers interested in commercializing the design,
which could be adapted for environmental monitoring and other uses.


========================================================================== Story Source: Materials provided by American_Chemical_Society. Note:
Content may be edited for style and length.


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Link to news story: https://www.sciencedaily.com/releases/2020/08/200817104319.htm

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