Act of sabotage determines mammalian embryonic development
Developmentally-programmed molecular failure uncovered in mammalian
embryos

Date:
April 13, 2022
Source:
Center for Genomic Regulation
Summary:
Alternative splicing is a fundamental biological process that
allows cells to make many different types of mRNAs and proteins
from a limited number of genes. For many animals, including humans,
it is a feature that is essential for the development of complex
cells such as muscles or neurons. A new study finds evidence that
the regulation of alternative splicing, which rarely goes wrong in
healthy cells, goes haywire in an unexpected place -- the cells of
a newly formed embryo. The findings could pave the way for advances
in assisted reproductive technologies and stem cell biology.



FULL STORY ========================================================================== Alternative splicing is a fundamental biological process that allows
cells to make many different types of mRNAs and proteins from a limited
number of genes.

For many animals, including humans, it is a feature that is essential
for the development of complex cells such as muscles or neurons.


==========================================================================
Its fundamental importance means that alternative splicing is a very
tightly regulated process. But a new study published today in the journal Science Advances has found evidence that the regulation of alternative splicing, which rarely goes wrong in healthy cells, goes haywire in an unexpected place -- the cells of a newly formed embryo.

Researchers at the Centre for Genomic Regulation (CRG) in Barcelona
made the discovery after creating an atlas of splicing events during
the early development of cows, humans and mice.

They found that when human embryos are balls of just 8 cells, they
express a huge variety of alternative mRNAs, so much so that the splicing diversity was the highest ever recorded across any cell or tissue studied
to date. When the embryos transitioned to the next stage of development,
their splicing activity returned to normal.

According to the authors of the study, this is evidence that the
regulation of alternative splicing collapses temporarily at a crucial
stage of development known as zygotic genome activation. This is when an
early embryo transitions from using maternal resources such as proteins
and RNA and making its own.

Importantly, the researchers believe the newly-discovered phenomenon
occurs because it is developmentally programmed -- a purposeful act of sabotage. "We think this happens because there are instructions in our
genome that tell a few genes to not do their job at that developmental
stage. The embryo cells mess up their splicing on purpose and they do so
for a functional reason," says ICREA Research Professor Manuel Irimia,
senior author of the study.

An important clue for why the regulation of splicing fails at this crucial moment lies in the function of the proteins affected. The researchers
found that splicing failure destroyed proteins responsible for responding
to DNA damage.

"We saw that the DNA damage response at this stage of development was low.

While splicing failure isn't the only factor affecting this defense
mechanism, it's partly responsible for destroying the proteins
involved. We don't know why this happens, but it's possibly because transcription itself carries a risk of DNA damage. As embryos activate
their genome for the first time and start to transcribe, there may
be trade-offs involved in order to avoid developmental failure." says
Dr. Barbara Pernaute, postdoctoral researcher at the CRG and co- first
author of the study.

According to Dr. Pernaute, these results improve our understanding of
how embryos develop during these early stages and could open doors for improvements in assisted reproductive technologies.

The findings could also be useful for advancing research efforts in the creation of totipotent cells from stem cells, a long-term aspiration
for regenerative medicine. As these early embryonic cells are truly
totipotent cells, knowledge of the mechanism could lead to advances that reverse engineer stem cells to induce totipotency.

"Recent studies carried out by other research groups around the world
have shown that artificially inducing the mechanism we find in our study transforms stem cells into totipotent cells. We believe these programmed splicing failures also occur in other physiological contexts. We are
only just scratching the surface for the importance this mechanism has
for biological processes," concludes Dr. Irimia.


========================================================================== Story Source: Materials provided by Center_for_Genomic_Regulation. Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Christopher D. R. Wyatt, Barbara Pernaute, Andre' Gohr, Marta Miret-
Cuesta, Lucia Goyeneche, Quirze Rovira, Marion C. Salzer,
Elvan Boke, Ozren Bogdanovic, Sophie Bonnal, Manuel Irimia. A
developmentally programmed splicing failure contributes to
DNA damage response attenuation during mammalian zygotic
genome activation. Science Advances, 2022; 8 (15) DOI:
10.1126/sciadv.abn4935 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2022/04/220413141530.htm

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