Imaging method highlights new role for cellular 'skeleton' protein
Molecules of actin help control the division of mitochondria, with implications for disease

Date:
August 10, 2020
Source:
Salk Institute
Summary:
While your skeleton helps your body to move, fine skeleton-like
filaments within your cells likewise help cellular structures to
move. Now, researchers have developed a new imaging method that
lets them monitor a small subset of these filaments, called actin.



FULL STORY ========================================================================== While your skeleton helps your body to move, fine skeleton-like filaments within your cells likewise help cellular structures to move. Now, Salk researchers have developed a new imaging method that lets them monitor
a small subset of these filaments, called actin.


========================================================================== "Actin is the most abundant protein in the cell, so when you image it,
it's all over the cell," says Uri Manor, director of Salk's Biophotonics
Core facility and corresponding author of the paper. "Until now, it's
been really hard to tell where individual actin molecules of interest
are, because it's difficult to separate the relevant signal from all
the background." With the new imaging technique, the Salk team has been
able to home in on how actin mediates an important function: helping the cellular "power stations" known as mitochondria divide in two. The work,
which appeared in the journal Nature Methods on August 10, 2020, could
provide a better understanding of mitochondrial dysfunction, which has
been linked to cancer, aging, and neurodegenerative diseases.

Mitochondrial fission is the process by which these energy-generating structures, or organelles, divide and multiply as part of normal
cellular maintenance; the organelles divide not only when a cell itself
is dividing, but also when cells are under high amounts of stress or mitochondria are damaged.

However, the exact way in which one mitochondrion pinches off into two mitochondria has been poorly understood, particularly how the initial constriction happens. Studies have found that removing actin from a cell entirely, among many other effects, leads to less mitochondrial fission, suggesting a role for actin in the process. But destroying all the actin
causes so many cellular defects that it's hard to study the protein's
exact role in any one process, the researchers say.

So, Manor and his colleagues developed a new way to image actin. Rather
than tag all the actin in the cell with fluorescence, they created an
actin probe targeted to the outer membrane of mitochondria. Only when
actin is within 10 nanometers of the mitochondria does it attach to the
sensor, causing the fluorescence signal to increase.

Rather than see actin scattered haphazardly around all mitochondrial
membranes, as they might if there were no discrete interactions between
actin and the organelles, Manor's team saw bright hotspots of actin. And
when they looked closely, the hotspots were located at the same locations
where another organelle called the endoplasmic reticulum crosses the mitochondria, previously found to be fission sites. Indeed, as the team
watched actin hotspots light up and disappear over time, they discovered
that 97 percent of mitochondrial fission sites had actin fluorescing
around them. (They speculate that there was also actin at the other 3
percent of fission sites, but that it wasn't visible).

"This is the clearest evidence I've ever seen that actin is accumulating
at fission sites," says Cara Schiavon, co-first author of the paper and
a joint postdoctoral fellow in the labs of Uri Manor and Salk Professor
Gerald Shadel.

"It's much easier to see than when you use any other actin marker."
By altering the actin probe so that it attached to the endoplasmic
reticulum membrane rather than the mitochondria, the researchers were
able to piece together the order in which different components join
the mitochondrial fission process. The team's results suggest that the
actin attaches to the mitochondria before it reaches the endoplasmic
reticulum. This lends important insight towards how the endoplasmic
reticulum and mitochondria work together to coordinate mitochondrial
fission.

In additional experiments described in a pre-print manuscript available
on bioRxiv, Manor's team also reports that the same accumulation of
endoplasmic reticulum-associated actin is seen at the sites where other cellular organelles -- including endosomes, lysosomes and peroxisomes -- divide. This suggests a broad new role for a subset of actin in organelle dynamics and homeostasis (physiological equilibrium).

In the future, the team hopes to look at how genetic mutations known to
alter mitochondrial dynamics might also affect actin's interactions with
the mitochondria. They also plan to adapt the actin probes to visualize
actin that's close to other cellular membranes.

"This is a universal tool that can now be used for many different applications," says Tong Zhang, a light microscopy specialist at Salk
and co- first author of the paper. "By switching out the targeting
sequence or the nanobody, you can address other fundamental questions
in cell biology." "We're in a golden age of microscopy, where new
instruments with ever higher resolution are always being invented;
but in spite of that there are still major limitations to what you can
see," says Manor. "I think combining these powerful microscopes with
new methods that select for exactly what you want to see is the next
generation of imaging."

========================================================================== Story Source: Materials provided by Salk_Institute. Note: Content may
be edited for style and length.


========================================================================== Journal Reference:
1. Cara R. Schiavon, Tong Zhang, Bing Zhao, Andrew S. Moore, Pauline
Wales,
Leonardo R. Andrade, Melissa Wu, Tsung-Chang Sung, Yelena Dayn,
Jasmine W. Feng, Omar A. Quintero, Gerald S. Shadel, Robert Grosse,
Uri Manor.

Actin chromobody imaging reveals sub-organellar actin
dynamics. Nature Methods, 2020; DOI: 10.1038/s41592-020-0926-5 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2020/08/200810113200.htm

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