Discovery could lead to more potent garlic, boosting flavor and bad
breath

Date:
August 5, 2020
Source:
Virginia Tech
Summary:
Their work could boost the malodorous - yet delicious -
characteristics that garlic-lovers the world over savor.



FULL STORY ==========================================================================
For centuries, people around the world have used garlic as a spice,
natural remedy, and pest deterrent -- but they didn't know how powerful
or pungent the heads of garlic were until they tasted them.


==========================================================================
But what if farmers were able to grow garlic and know exactly how potent
it would be? What if buyers could pick their garlic based on its might?
A team of Virginia Tech researchers recently discovered a new step in
the metabolic process that produces the enzyme allicin, which leads to
garlic's delectable flavor and aroma, a finding that upends decades of
previous scientific belief. Their work could boost the malodorous --
yet delicious - - characteristics that garlic-lovers the world over savor.

"This information changes the whole story about how garlic could be
improved or we could make the compounds responsible of its unique
flavor," said Hannah Valentino, a College of Agriculture and Life
Sciences Ph.D. candidate. "This could lead to a new strain of garlic
that would produce more flavor." The discovery of this pathway opens
the door for better control of production and more consistent crops,
which would help farmers. Garlic could be sold as strong or weak,
depending on consumer preferences.

The research was recently published in the Journal of Biological
Chemistry.



==========================================================================
When Valentino, an Institute for Critical Technology and Applied Science doctoral fellow, and her team set out to test the generally accepted
biological process that creates allicin, they found it just didn't happen.

That's when the team of researchers set out to discover what was really happening in garlic.

As they peeled back the layers, they realized there was no fuel to power
the previous accepted biological process that creates allicin.

"By using rational design, Hannah found a potential substrate," said
Pablo Sobrado, professor of biochemistry in the College of Agriculture
and Life Sciences and a member of the research team. "This is significant because by finding the metabolic pathway and understanding how the enzyme actually works and its structure gives us a blueprint of how allicin is
created during biosynthesis." Valentino and the team -- which included undergraduate students -- worked in the Sobrado Lab in the Fralin Life
Sciences Institute directly with the substrates that comprise garlic,
doing their work solely in vitro.



========================================================================== Hannah Valentino, left, and Pablo Sobrado, right, are conducting research
that is laying the foundation for a future in which buyers can choose
garlic based on its strength and flavor profile.

The researchers found that allicin, the component that gives garlic its
smell and flavor, was produced by an entirely different biosynthetic
process. Allyl- mercaptan reacts with flavin-containing monooxygenase,
which then becomes allyl-sulfenic acid.

Importantly, the allicin levels can be tested, allowing farmers
to know the strength of their crops without the need for genetic
engineering. Greater flavor can simply be predicted, meaning powerful
garlic could simply be bred or engineered.

"We have a basic understanding of the biosynthesis of allicin that it is involved in flavor and smell, but we also now understand an enzyme that
we can try to modulate, or a modify, to increase or decrease the level of
the flavor molecules based on these biological processes," Sobrado said.

Because of their work, the future awaits for fields of garlic harsh
enough to keep even the most terrifying vampires at bay.


========================================================================== Story Source: Materials provided by Virginia_Tech. Original written by
Max Esterhuizen. Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Hannah Valentino, Ashley C. Campbell, Jonathan P. Schuermann,
Nazneen
Sultana, Han G Nam, Sophie LeBlanc, John J. Tanner, Pablo Sobrado.

Structure and function of a flavin-dependent S-monooxygenase from
garlic (Allium sativum). Journal of Biological Chemistry, 2020;
jbc.RA120.014484 DOI: 10.1074/jbc.RA120.014484 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2020/08/200805110115.htm

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