Recipe for success -- interaction proteomics become a household item


Date:
August 11, 2020
Source:
University of Helsinki
Summary:
A research team introduces a new optimized and integrated
interaction proteomics protocol that combines two state-of-the
art methods to allow rapid identification of protein-protein
interactions and more.



FULL STORY ========================================================================== Proteins in human cells do not function in isolation and
their interactions with other proteins define their cellular
functions. Therefore, detailed understanding of protein-protein
interactions (PPIs) is the key for deciphering regulation of cellular
networks and pathways, in both health and disease.


==========================================================================
In a study published in the of September issue (advanced online
10th of August) of Nature Protocols, a research team led by Research
Director Markku Varjosalo from the Institute of biotechnology & HiLIFE, University of Helsinki, introduces an optimised and integrated interaction proteomics protocol (named MAC-tag technology). The protocol combines
two state-of-the art methods affinity purification -- mass spectrometry
(AP-MS) and proximity-dependent biotin identification (BioID to allow
rapid identification of protein-protein interactions and more.

The MAC-tag technology allows an easy way to probe the molecular
level localisation of protein of interest. The developed MAC-tag
and the integrated approach will empower, not only the interaction
proteomics community, but also cell/molecular/structural biologists,
with an experimentally proven integrated workflow for mapping in detail
the physical and functional interactions and the molecular context
of proteins.

"The MAC-tag technology stems from long-term efforts on developing
new systems biology tools for systematically studying the molecular interactions of proteins. The identification of protein-protein
interactions and their changes in disease settings, such as cancer,
has proven in our hands a powerful tool and has allowed us to find
exact molecular mechanisms underlying these diseases. In principle,
our protocol can be used in so many different ways that we probably have
not even envisioned half of them." Dr. Varjosalo states.

The MAC-tag technology is currently in use by Dr. Varjosalo and his
consortia of virologists, medicinal chemists and other 'omics' researchers
in search for novel druggable host proteins as therapeutic targets to
inhibit the SARS-CoV- 2 infection and therefore to fight Covid-19.


========================================================================== Story Source: Materials provided by University_of_Helsinki. Note:
Content may be edited for style and length.


========================================================================== Journal Reference:
1. Xiaonan Liu, Kari Salokas, Rigbe G. Weldatsadik, Lisa Gawriyski,
Markku
Varjosalo. Combined proximity labeling and affinity
purification-mass spectrometry workflow for mapping and visualizing
protein interaction networks. Nature Protocols, 2020; DOI:
10.1038/s41596-020-0365-x ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2020/08/200811120107.htm

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