Forging molecular bonds with green light

Date:
August 24, 2020
Source:
Queensland University of Technology
Summary:
Scientists have created a new molecular coupling tool employing
both green light and pH triggers that has potential for use in
applications such as drug delivery and 3D cell culture platforms.



FULL STORY ========================================================================== Queensland University of Technology (QUT) researchers have created a new molecular coupling tool employing both green light and pH triggers that
has potential for use in applications such as drug delivery and 3D cell
culture platforms.


========================================================================== Their research has been published in the journal Nature Communications.

* The researchers designed photoreactive molecules that enabled
them to
couple together, using green light as the fuel, polymers commonly
used in medical and industrial applications. They then controlled
the molecules' photoreactivity by changing pH (the measure of how
acid or alkaline a substance is).

* It is the first time pH has been used as an on/off switch for
a green
light-activated, catalyst-free chemical process.

* The green light used was also the longest wavelength of light
(up to 500
nanometres) employed to date to control a catalyst-free
photochemical bond-forming reaction.

* To demonstrate the application potential of this photochemical
innovation, the team produced a range of hydrogels with varying
mechanical properties. Hydrogels are commonly used in contact
lenses, tissue engineering scaffolds, as drug delivery carriers,
and for cell biology studies.

The research was conducted by lead author and QUT PhD chemistry researcher Kubra Kalayci, Australian Research Council (ARC) DECRA Fellow Dr Hendrik Frisch, Research Fellow Dr Vinh Truong, and ARC Laureate Fellow Professor Christopher Barner-Kowollik from QUT's Soft Matter Materials Laboratory
in the Science and Engineering Faculty Centre for Materials Science.

Professor Barner-Kowollik said scientists were constantly seeking to move further away from using harsh UV light to activate chemical reactions.

"Our photochemical innovation is another example of what is called
red-shifting -- moving through the colours of light in the spectrum, from
blue to green towards red, to light that has longer wavelengths," he said.

"In the past, most of these types of photochemical reactions were
triggered by harsh UV (ultra-violet) light. But that prevents applications
in a biological context because UV light has so much energy it kills
cells.



========================================================================== "Dentistry is an example of one of the areas that has shifted. Initially dentists were using UV lamps. Now anyone who has had a filling probably
knows that the dentist uses a little lamp with longer wavelength blue
light for curing.

"The longer the wavelength of light the better, in principle. The
radiation is less harmful, so it can be used for biological applications,
and it allows for deeper light penetration. For dentistry, that means
better and more uniform curing.

"But it is also more difficult to do, because the longer the wavelength
of light the less energy you have to drive the chemical reaction.

"Adding an additional stimulus with the green light, such as we have with varying the pH as a reversible on-off switch for the reaction, provides
the opportunity for better regulation. This is especially important
for drug delivery systems, where the drug needs to be released under a
specific pH, as pH varies throughout the human body.

"This is also a catalyst-free reaction. It means there's no helper
molecule to make it happen. That's important for biological application
as well because in many cases helper molecules contain metal, and you
don't want something that could leach out, or something that is found to
be cytotoxic or carcinogenic." To investigate the new green light-pH
coupling tool's suitability for biomaterials engineering, Ms Kalayci
said the research team created hydrogels with different properties.



========================================================================== "These showed that green light allowed higher penetration depths,
resulting in fabrication of thicker hydrogels," she said.

Dr Truong said cells cultured inside the hydrogels "showed the process
for creating the gels was non-toxic, and the cells also remained viable
for several days." The team believes the new coupling tool has a range
of other potential applications.

"For example, in the context of personalised medicine," Dr Truong and
Dr Frisch said. "You might want to use our reaction to attach a cancer
drug to a specific part of a molecule to deliver the drug in a way
that is suited to a particular patient." Professor Barner-Kowollik
said it was also another step towards achieving "molecular surgery."
"What chemists hope to do is be able to 'operate' on one part of a
molecule without affecting anything else," he said.

"So, for example, if you had a protein, a large complex molecule, we'd
like to be able to use light like a chemical scalpel and very delicately
go in and change part of that molecule without affecting any other
part. That provides many potential applications." Applications could
include, Dr Truong said, "looking at the selective crosslinking of DNA
to study the underlying mechanism of a cancer, looking for avenues for
targeted treatment, or creating dynamic hydrogel scaffolds to study cell interactions for tissue regeneration therapy.

"Using light, we are providing chemical tools to be able to achieve
these aims."

========================================================================== Story Source: Materials provided by
Queensland_University_of_Technology. Note: Content may be edited for
style and length.


========================================================================== Journal Reference:
1. Kubra Kalayci, Hendrik Frisch, Vinh X. Truong, Christopher Barner-
Kowollik. Green light triggered [2 2] cycloaddition of halochromic
styrylquinoxaline--controlling photoreactivity by pH. Nature
Communications, 2020; 11 (1) DOI: 10.1038/s41467-020-18057-9 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2020/08/200824092025.htm

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