Glycans in the SARS-CoV-2 spike protein play active role in infection


Date:
September 23, 2020
Source:
American Chemical Society
Summary:
Many efforts to develop vaccines and therapies to thwart
SARS-CoV-2 focus on the coronavirus spike protein, which binds the
angiotensin-converting enzyme 2 (ACE2) on human cells to allow
viral entry. Now, researchers have uncovered an active role for
glycans -- sugar molecules that can decorate proteins -- in this
process, suggesting targets for vaccines and therapies.



FULL STORY ==========================================================================
As the COVID-19 pandemic rages on, researchers are working overtime to
develop vaccines and therapies to thwart SARS-CoV-2, the virus responsible
for the disease Many efforts focus on the coronavirus spike protein,
which binds the angiotensin-converting enzyme 2 (ACE2) on human cells
to allow viral entry.

Now, researchers reporting in ACS Central Science have uncovered an
active role for glycans -- sugar molecules that can decorate proteins --
in this process, suggesting targets for vaccines and therapies.


========================================================================== Before the SARS-CoV-2 spike protein can interact with ACE2 on a human
cell, it changes shape to expose its receptor binding domain (RBD),
the part of the protein that interacts with ACE2. Like many viral
proteins, the SARS-CoV- 2 spike protein has a thick coat of glycans on
its surface. These glycans, which are attached at specific sites, help
shield the viral proteins from the host immune system. Rommie Amaro and colleagues at University of California San Diego, Maynooth University
(Ireland) and the University of Texas at Austin wondered whether certain glycans in the SARS-CoV-2 spike protein might also be active players in
the process leading to infection.

To find out, the researchers used structural and glycomic data to
build molecular dynamics simulations of the SARS-CoV-2 spike protein
embedded in the viral membrane. The computer models, which presented a
detailed snapshot of every atom in the spike glycoprotein, revealed that N-glycans linked to the spike protein at certain sites (N165 and N234)
helped stabilize the shape change that exposes the RBD, which could help promote infection. The simulations also identified regions of the spike
protein that weren't coated by glycans and thus could be vulnerable to antibodies, especially after the shape change. In laboratory experiments
using biolayer interferometry, the team showed that mutating the spike
protein so that it no longer had glycans at N165 and N234 reduced binding
to ACE2. These results lay the foundation for new strategies to fight
the pandemic threat, the researchers say.

The authors acknowledge funding from the National Institutes of Health,
the National Science Foundation, the Research Corporation for Science Advancement, UC San Diego Moores Cancer Center, the Irish Research
Council, and the Visible Molecular Cell Consortium.


========================================================================== Story Source: Materials provided by American_Chemical_Society. Note:
Content may be edited for style and length.


========================================================================== Journal Reference:
1. Lorenzo Casalino, Zied Gaieb, Jory A. Goldsmith, Christy K. Hjorth,
Abigail C. Dommer, Aoife M. Harbison, Carl A. Fogarty, Emilia
P. Barros, Bryn C. Taylor, Jason S. McLellan, Elisa Fadda,
Rommie E. Amaro. Beyond Shielding: The Roles of Glycans in
the SARS-CoV-2 Spike Protein. ACS Central Science, 2020; DOI:
10.1021/acscentsci.0c01056 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2020/09/200923090426.htm

--- up 4 weeks, 2 days, 6 hours, 50 minutes
* Origin: -=> Castle Rock BBS <=- Now Husky HPT Powered! (1337:3/111)