Researchers find new way to protect plants from fungal infection

Date:
September 22, 2020
Source:
Martin-Luther-Universita"t Halle-Wittenberg
Summary:
Widespread fungal disease in plants can be controlled with a
commercially available chemical that has been primarily used in
medicine until now. In a comprehensive experiment scientists have
uncovered a new metabolic pathway that can be disrupted with this
chemical, thus preventing many known plant fungi from invading
the host plant.



FULL STORY ========================================================================== Widespread fungal disease in plants can be controlled with a commercially available chemical that has been primarily used in medicine until
now. This discovery was made by scientists from Martin Luther University Halle-Wittenberg (MLU) and the University of the State of Parana'
in Brazil. In a comprehensive experiment the team has uncovered a
new metabolic pathway that can be disrupted with this chemical, thus
preventing many known plant fungi from invading the host plant. The team reported on their study in the scientific journal Phytopathology.


==========================================================================
The fungus Colletotrichum graminicola is prevalent around the world. It
infects maize, causing anthracnose, a disease that causes the plant's
leaves to turn yellow at first and then ultimately to succumb to
toxins. The fungus multiplies through spores that initially land on the
surface of the plant. There they find rather inhospitable conditions: a
lack of most of the nutrients that fungi need to develop -- in particular nitrogen. "The only option they have is to break down some of their own nitrogen-containing molecules, for instance purines, the building blocks
of DNA or RNA," explains plant pathologist Professor Holger Deising
from MLU.

The researchers on Deising's team have found a way to impede this
transitional phase which the fungus relies on. To do this, the team administered acetohydroxamic acid onto the plants, a substance also used
to treat harmful bacteria in the human stomach, and which is known to
inhibit the breakdown of urea. "The acid prevents the harmful fungi from penetrating into the plants and from becoming infectious," says Deising.

The team also tested whether the findings from C. graminicola and maize
could be transferred to other plants and fungi. The acid was also
found to be effective against numerous other pathogens which cause,
for example, powdery mildew in cereal crops, late blight in potatoes,
as well as corn and bean rust.

"We have been able to develop a completely new approach to pathogen
control that uses an existing active ingredient and thus can be quickly
used commercially," says Deising.

The scientists conducted extensive experiments in order to come to their conclusions. They wanted to understand the molecular details of how the
fungus manages to obtain nitrogen at the onset of the infection. First,
they generated a series of random mutations in the genome of the fungus
C. graminicola. "Then we inoculated the different fungal mutants onto the plants to see which ones were no longer infectious," says Deising. One of
these mutants had a defect in the purine degradation pathway. In order
to check whether the mutants' failure to infect the plant was actually
caused by a lack of nitrogen, the researchers then applied nitrogen to
the plants. "Once nitrogen was added, even the harmless mutants became infectious again," says Deising. The team was able to induce the same
defect they had observed in the mutants in wildtype fungi by applying acetohydroxamic acid because it blocks the purine degradation pathway,
too.


========================================================================== Story Source: Materials provided by Martin-Luther-Universita"t_Halle-Wittenberg. Note: Content may be edited
for style and length.


========================================================================== Journal Reference:
1. Elvio Henrique Benatto Perino, Chirlei Glienke, Alan de Oliveira
Silva,
Holger B. Deising. Molecular Characterization of the Purine
Degradation Pathway Genes ALA1 and URE1 of the Maize Anthracnose
Fungus Colletotrichum graminicola Identified Urease as a Novel
Target for Plant Disease Control. Phytopathology(R), 2020; 110
(9): 1530 DOI: 10.1094/ PHYTO-04-20-0114-R ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2020/09/200922102427.htm

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